LOCUS       40924_19016        3470 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pF12A RM Flexi, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Vidugiriene J, Schenborn E, McNamara B.
  TITLE     Regulated Mammalian Expression System Technical Manual, TM289
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Vidugiriene J, Schenborn E, McNamara B.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2006) Scientific Communications, Promega 
            Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 3470)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3470)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-SEP-2006) Scientific Communications, Promega Corporation, 2800 
            Woods Hollow Road, Madison, WI 53711, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3470
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    16..309
                     /label=12X lambda operator
                     /note="12X lambda operator"
     regulatory      322..434
                     /label=minimal CMV promoter
                     /note="minimal CMV promoter"
                     /regulatory_class="promoter"
     CDS             477..809
                     /label=barnase
                     /note="ribonuclease from Bacillus amyloliquefaciens"
     misc_feature    822..877
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     polyA_signal    complement(982..1103)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1350..1454
                     /label=AmpR promoter
     CDS             1455..2312
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      complement(2473..3061)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3177..3460
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to
                     maintain plasmids as monomers"
ORIGIN
        1 agtactccgc tcgagtttac ctctggcggt gatagtcgag tttacctctg gcggtgatag
       61 tcgagtttac ctctggcggt gatagtcgag tttacctctg gcggtgatag tcgagtttac
      121 ctctggcggt gatagtcgag tttacctctg gcggtgatag tcgagtttac ctctggcggt
      181 gatagtcgag tttacctctg gcggtgatag tcgagtttac ctctggcggt gatagtcgag
      241 tttacctctg gcggtgatag tcgagtttac ctctggcggt gatagtcgag tttacctctg
      301 gcggtgatag tcgactctag ataggcgtgt acggtgggag gcctatataa gcagagctgg
      361 tttagtgaac cgtcagatcc ctggagacgc catccacgct gttttgacct ccatagaaga
      421 caccgggacc gatcaaccta agcttgcgat cgccatggaa taagtaagga atccacatgg
      481 cacaggttat caacacgttt gacggggttg cggattatct tcagacatat cataagctac
      541 ctgataatta cattacaaaa tcagaagcac aagccctcgg ctgggtggca tcaaaaggga
      601 accttgcaga cgtcgctccg gggaaaagca tcggcggaga catcttctca aacagggaag
      661 gcaaactccc gggcaaaagc ggacgaacat ggcgtgaagc ggatattaac tatacatcag
      721 gcttcagaaa ttcagaccgg attctttact caagcgactg gctgatttac aaaacaacgg
      781 accattatca gacctttaca aaaatcagat aatgtttaaa cgaattcggg ctcggtaccc
      841 ggggatcctc tagagtcgac ctgcaggcat gcaagctgat ccggctgcta acaaagcccg
      901 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc
      961 ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     1021 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     1081 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     1141 agggggagat gtgggaggtt tttttaagca agtaaaacct ctacaaatgt ggtaaaatcg
     1201 aattctaatg gatcctcttt gcgcttgcgt tttcccttgt ccagatagcc cagtagctga
     1261 cattcatccg gggtcagcac cgtttctgcg gactggcttt ctacgtaatg gtttcttaga
     1321 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     1381 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     1441 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     1501 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     1561 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     1621 agagttttcg ccccgaagaa cgttttccaa tgatgagcac tttcaaagtt ctgctatgtg
     1681 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     1741 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     1801 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     1861 ttctgacaac tatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     1921 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     1981 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     2041 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     2101 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     2161 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     2221 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     2281 ctgagatagg tgcctcactg attaagcatt ggtaattcga aatgaccgac caagcgacgc
     2341 ccaaccggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     2401 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2461 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2521 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2581 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2641 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2701 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2761 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2821 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2881 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     2941 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     3001 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatttca
     3061 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     3121 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt tatagtccgg
     3181 aaatacagga acgcacgctg gatggccctt cgctgggatg gtgaaaccat gaaaaatggc
     3241 agcttcagtg gattaagtgg gggtaatgtg gcctgtaccc tctggttgca taggtattca
     3301 tacggttaaa atttatcagg cgcgattgcg gcagtttttc gggtggtttg ttgccatttt
     3361 tacctgtctg ctgccgtgat cgcgctgaac gcgttttagc ggtgcgtaca attaagggat
     3421 tatggtaaat ccacttactg tctgccctcg tagccatcga gataaaccgc
//
